After 30 minutes of incubation at 37 C in the dark, the percentage of PI positive cells was determined by using a flow cytometer (BD FACSCanto II, BD Biosciences), with 488 nm excitation laser and 585/42 nm band pass filter
Determining the total glutathione and amount of GSSG are performed in parallel reactions
The intrinsic pathway (mitochondrial pathway) involves two primary mechanisms of apoptosis: cytochrome c (Cyt C) release and the opening of the mitochondrial permeability transition pore (MPTP) (Taneja et al., 2001)
Ferroptosis in cancer therapy: a novel approach to reversing drug resistance
The processes involved in sperm handling, including washing, centrifugation, and cryopreservation, often expose sperm to elevated levels of ROS, which can compromise sperm viability and DNA integrity [60]