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To ensure the absorbance from oil red O staining was within the linear detection range of the plate reader, a calibration curve was established for oil red O staining in adipocytes using a previously described protocol.[23] 2.12

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MOTS-C (Mitochondrial Open-Reading-Frame of the 12S rRNA-C) is a mitochondrial-derived peptide that plays a crucial role in metabolic regulation

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Because subcutaneous fat depth varies enormously between individuals the post cites a range from roughly 5 mm to over 25 mm depending on age, sex, body composition, and the site being used