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Samples were mixed for 5 min and centrifuged again at 2000 rpm for an additional 5 min

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Allylamine Allylamines, such as naftifine, butenafine, and terbinafine, inhibit a flavine adenine dinucleotide dependent enzyme, squalene epoxidase (EC 1.14.99.7) [171], responsible for converting squalene to squalene-2,3-epoxide, an intermediate in the biosynthesis of lanosterol [163, 172]