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Optimal grids for AMYR:Cagri complexes and CTR: Cagri ( N = 1) were imaged by conventional cryo-TEM on a Thermo Fisher Scientific Titan Krios microscope operated at an accelerating voltage of 300 kV with a 50 m C2 aperture and a 100 m objective aperture at an indicated magnification of 105kx in nanoprobe EFTEM mode, yielding a calibrated pixel sampling of 0.85 .pix -1

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Use in Insomnia Mouse Model: The three proteins that were successfully expressed and confirmed by their molecular weight and fluorescence (DSIP-CBBBP, CBBBP, and DSIP) were used in the insomnia mouse model