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This first reaction is the rate-limiting step in the synthesis of GSH and is regulated by cysteine availability

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Reduction in appetite sensations within a 14-day period, greater level of satiety and less desire to eat

Foyer CH, Noctor G (2009) Redox Regulation in Photosynthetic organisms

The bound ligand was separated from free ligand by filtration through a Perkin Elmer Unifilter-96 GF/C 96-well microplate using the Perkin Elmer Unifilter-96 Harvester (Perkin Elmer, Waltham, MA), followed by three washes with ice-cold assay buffer