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The downstream analysis was carried out using the free software environment R version 4.0.3 and the open-source integrated development environment (IDE) RStudio ( v1.4.1103, and the environmental contaminants were excluded using the open-source decontam R package ( ( phyloseq package ( adonis2 in the vegan R package, was performed to determine whether there was a significant separation between different sample groups ( accessed on 22 December 2023)

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J Med Virol (2020) 92