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In vitro protein acyltransferase (PAT) assay Palmitoyl acyltransferase constituted by purified recombinant DHHC9 and GCP16 proteins (100 ng for each) were incubated with GLUT1 (2 g) in 25 l of reaction buffer (50 mM Tris-HCl [pH 7.4], 10 M palmitoyl alkyne-CoA, 1 M palmostatin B) at 25 C for 1 h followed by a Cu(I)-assisted click reaction with biotin picolyl azide (50 M) to biotinylate the proteins with the incorporation of palmitoyl alkyne

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This interaction leads to fast activation and modulation of cellular events, promoting vascular formation and recovery from vascular injuries

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Oksidatif stresi azaltarak ve mitokondriyal fonksiyonu destekleyerek, enerji seviyelerini artrabilir ve gn boyunca optimum aktivite seviyelerinin korunmasna yardmc olabilir