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Withanolides are known to engage with GABAergic and serotonergic pathways, which mitigates the hyperactivation of the HPA axis, resulting in enhanced stress tolerance and anxiolytic effects (78)

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A: Repeated freeze-thaw cycles can cause peptide degradation and loss of bioactivity

Carry-over and period effects were investigated by comparing the baseline measurements at the beginning of each period (V1 and V4) as well as interpreting the beta coefficient from the interaction between the period and the assigned treatment

The FDA has not approved these peptides for obesity treatment (FDA guidance on unapproved peptides)