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The cell lysates were quantified using Pierce 660 nm Protein Assay Reagent (Thermo Fisher Scientific, 22660) and Ionic Detergent Compatibility Reagent for Pierce 660 nm Protein Assay Reagent (Thermo Fisher Scientific, 22663)

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Severe ASD has been linked to elevated levels of IL-1 and IL-4 in the neonatal blood (Theoharides et al., 2016), and increased serum levels of IL-1 and IL-6 compared to control groups ( Recent data support findings that immune cells activity in ASD subjects is compromised, with amplified inflammatory signaling and reactivity of immune cells such as B-cells, neutrophils and monocytes (Majerczyk et al., 2022)

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