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Urol Res 38(4):239247

DNA extraction was performed, and 500 ng of DNA was subjected to bisulfite conversion using the EZ DNA Methylation Kit from Zymo Research, following the manufacturers protocol

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Sample volumes were normalized to 35 L with EPPS buffer (200 mM, pH 8), diluted with HPLC grade CH 3 CN (9 L), and then labeled (5 L of 20 g/L per sample) with the corresponding TMTsixplex Isobaric Mass Tag (Themo Scientific, #90064B)

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