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Gut 73(4): 691702
doi:10.1056/NEJMoa2032183 2
TIPE2 attenuates neuroinflammation and brain injury through Bcl-2/Bax/cleaved caspase-3 apoptotic pathways after intracerebral hemorrhage in mice
The histological analysis was performed using a high power objective (x63) in the microscope ZEISS AXIOSKOP 2, by counting cells in three areas with a minimum of approximately 100 m 2 , using three distinct serial sections (50 m interval between sections) per animal, for five different animals
Visualization of mitochondria Mitochondria were identified by MitoTracker Red (Molecular Probes Inc., Eugene, OR, USA) staining [18] and analyzed with a Leica confocal laser scanning microscope using SCANware software (Leica Lasertechnik GmbH, Heidelberg, Germany)
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