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As can be seen from Figures 4 , the samples of NC, FI and GSH groups are all in the 95% confidence interval, and can be spatially separated uniformly without overlap, indicating that there are significant differences in metabolites between the two groups, and that they are able to be analyzed in the next step

10.1080/13813455.2018.1437186 91 SharmaR.BrownD.AwasthiS.YangY.SharmaA.PatrickB.et al (2004)

The reaction mixture was incubated in boiling water for 20 min and cooled to room temperature