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After 30 min, glucose transport across the membrane was assessed using glucose colorimetric detection

Similarly, oxidation of Met-36 and Met-48 in human stem cell factor (huSCF) derived from Escherichia coli decreased its potency 40% and 60% (respectively) while increasing the dissociation rate constant of SCF dimer by two- to threefold, which suggests an effect on subunit binding and tertiary structure (9)

Sterile water lacks preservative - bacteria can grow between uses