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Mutant generation The N-terminal 6xHis version of plasmid pSP19T7LTMGST1, containing wild-type rat MGST1 was used as the template for site-directed mutagenesis 47

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It participates in the conversion of homocysteine to methionine and the metabolism of methylmalonic acid, both crucial for energy production at the cellular level

Moreover, as discussed in the CY 2026 PFS proposed rule (90 FR 32664), section 1899(b)(2)(D) of the Act requires participating ACOs to include primary care ACO professionals that are sufficient for the number of Medicare FFS beneficiaries assigned to the ACO and that, at a minimum, the ACO shall have at least 5,000 such beneficiaries assigned to it under section 1899(c) of the Act in order to be eligible to participate in the Shared Savings Program