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Promoter #14 and SOD (pKLH167-ske-F/ske1717-SOD-R) were amplified from pKLH167 and ligated into the backbone of pKLH167 using a pair of primer pKLH167-ske-F/pKLH167-ske-R, yielding pKLH337
Treasure Island, FL, USA: 2023
Recovery from ROS-induced toxicity was tested through MTT assay, in HeLa and SHSY-5Y cells pre-exposed to 10 M 3-AT for 15 min or 1 mM MPP + for 24 h respectively, followed by 250 M 15 min Sp treatment
Glutathione travels through the body, neutralising these free radicals and preventing them from causing harm
Additionally, proteolysis targeting chimeras (PROTACs) offer a novel approach to modulate ferroptosis: PROTACs targeting ACSL4 or Nrf2 have been developed to either induce ferroptosis (in renal cell carcinoma) or enhance antioxidant defense (in CKD), respectively, with improved specificity compared to small-molecule inhibitors
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