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[PubMed ID: 1352530]

Per the manufacturers protocol, slides were incubated with the kit amplifier/enhancer for 20 min at room temperature (25C), followed by incubation with the HRP-conjugated secondary reagent for 30 min at room temperature

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269 In a pancreatic cell line, SIRT6 induces the production of cytokines including IL8 and TNF, which promote cell migration