is glp-1 r retatrutide Retatrutide, a GIP, and glucagon receptor agonist, for people with type 2 diabetes: a randomised, double-blind, placebo and active-controlled, parallel-group, phase 2 trial conducted in the USA Retatrutide for Weight Loss: TRIUMPH-1

TK and IA carried out the statistical analysis

Cell culture and treatment Human HK-2 cells (Cell Bank of the Chinese Academy of Science, Shanghai, China) were cultured in F12 medium with 10% FBS (Gibco, USA) and 1% penicillin/streptomycin (Beyotime, C0222) at 37 with 5% CO 2

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Two days later, GLP-1, dulaglutide, DCA, forskolin, L-glutamine, sodium butyrate, and 007-AM (8-pCPT-2-O-Me-cAMP-AM), as well as glucose in Hanks Balanced Salt Solution (HBSS) containing 0.01% (v/v) bovine serum albumin (BSA), 5-mM HEPES, and 0.5-mM 3-isobutyl-1-methylxanthine (IBMX), were applied to the cells at a volume of 50 L per well and incubated for 60 min