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The supernatant from the hypotonic solution was combined with the supernatant from the hypertonic solution, centrifuged to remove debris, and dialyzed against 20LmM Tris, pH 7.5 and 150LmM NaCl (Tris-buffered saline, TBS) overnight at 4 C

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Synthesis & Quality Assurance Synthesis Overview GHK is produced using solid-phase peptide synthesis (SPPS), enabling controlled assembly of the tripeptide sequence with high fidelity