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cAMP accumulation assay Wild-type GLP-1R and GLP-1R mutants were cloned into the expression receptor pcDNA3.1/V5-His-TOPO vector (Invitrogen) at the HindIII and EcoRI sites by using the QuickChange site-directed mutagenesis and a flag-tag was inserted after native signal sequence (see primers in Supplementary Table 2)

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7 Allergy Department, Hospital Universitario La Paz, Madrid, Spain

Las dosificaciones que se recogen en los estudios que arrojan resultados positivos en la utilizacin de grasa, mejora de la energa, mejor recuperacin etc

Regenerative and Protective Actions of the GHK-Cu Peptide in the Light of the New Gene Data Share