Key Research Areas for Glutathione Intracellular redox status measurement GSH/GSSG ratio as a primary cellular oxidative stress index Oxidative stress induction and rescue assays GSH depletion (buthionine sulfoximine, BSO) and repletion studies Glutathione peroxidase (GPx) and glutathione reductase (GR) enzyme activity assays Glutathione S-transferase (GST) detoxification pathway research xenobiotic and electrophile conjugation studies S-glutathionylation and redox-regulated post-translational modification studies Nrf2/ARE pathway research transcriptional regulation of GSH synthesis and antioxidant gene expression Mitochondrial GSH pool studies mtGSH depletion, mitochondrial oxidative damage, and mtDNA protection research Cancer cell biology GSH-dependent drug resistance, ferroptosis pathway research, GSH-targeted therapeutic strategy studies Neurodegeneration pathway research GSH depletion in dopaminergic and other neuronal cell models Immune cell function GSH regulation of T-cell proliferation, cytokine production, and inflammatory signalling Lipid peroxidation and ferroptosis pathway studies GPx4-GSH axis research GCL/GS enzyme activity and GSH biosynthesis regulation studies in vitro What Do Studies Say About Glutathione
K562 and KOPM28 cells were cultured in Iscoves Modified Dulbeccos Medium (IMDM, Sigma, I6529) with 10% FBS
The present review is an attempt to summarize the most important findings on GSTs in fungus-, bacterium- and virus-infected plants with a special attention to the possible functions of GSTs in disease resistance
The second-generation antiandrogens combined with ferroptosis activators can further inhibit tumor proliferation by inhibiting the expression of GPX4 [17]
In a recent study, pharmacological inhibition of NLRP3 inflammasome by MCC950 improved pathological damage and reduced inflammatory response in experimental pancreatitis ( 5 TCM used to relieve AP by targeting NLRP3 In Chinese medicine (CM), AP is classified under abdominal pain ( Futong ), spleen-heart pain ( Pi Xintong ), and pancreatic inflammation ( Yi Dan ) ( Gan Yu ), trauma, and invasion of exogenous pathogens ( Liu Yin ) (Wu, 2002), all of which may exacerbate the progression of AP
