After animals were killed by exsanguination at each time point, the livers were harvested and rinsed in ice-cold saline
An obstacle to conducting human clinical trials to test the effect of an intervention on length of life is limited by the duration of time it would take to confirm or refute the intervention being tested, and studying rodents or other models can practically provide a proof-of-concept because they can be done in a time-sensitive manner
NTS were purified by layering on 4 C Percoll gradient suspension containing Eagles minimum essential medium, penicillin-streptomycin (10,000 U per ml penicillin/10,000 U per ml streptomycin), and 1 M HEPES in 0.85% NaCl with cercariae suspension and centrifuged at 500 g for 15 min
Single-Dose Vial - Discard Unused Portion, Sterile Rx only Distributed by: Hospira, Inc
However, with the current research, the potential risks associated with even mild iodine excess during pregnancy warrant careful consideration