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After treatment, cells were immediately analyzed for apoptosis and the cell cycle using a BD FACSCelesta Cell Analyzer with an Annexin V-FITC/PI Apoptosis Detection Kit and PI/RNase Staining Buffer (BD Biosciences, Franklin Lakes, NJ, USA), respectively, according to the manufacturers instructions
Refrigeration of the product is not recommended
In an animal study, inhibition of the NF-B pathway led to a significant reduction in the expression of serum proinflammatory cytokines, including IL-6, IL-1 and TNF-, as well as an increase in the expression of the tight junction proteins occludin and ZO-1, thereby improving the function and structure of the intestinal mucosa
L-Carnitine is a non-essential amino acid found in high concentrations in human heart and liver tissue, where it participates in metabolizing fatty acids into energy by the mitochondria
10.3844/ajbbsp.2010.204.212 50
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