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Data analysis Graph Pad Prism software (Version 8) was used for data analysis

demonstrated that flow-mediated vasodilation and nitroglycerin-induced vasodilation are significantly reduced in patients with HFpEF compared to those without heart failure

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For lipid peroxide production measurements using C11- BODIPY (581/591) (2 mM) (Thermofischer, Waltham, MA), 2x10 5 cells were labeled with C11-BODIPY in 1 mL of warm complete medium for 10 min in a tissue culture incubator (37C, 5% CO 2 ) in the dark

After 30 days, when mice in both the HFD-SY and HFD-WM groups reached the weight range of lean age-matched control mice, treatment was withdrawn and the glucose-lowering efficacy of the interventions was determined by measuring hyperglycaemia, hyperinsulinaemia and insulin resistance