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Cell supernatants were added to 96-well plates to determine LDH release, and absorbance was measured at 490 nm using an automated ELISA reader (SpectraMax M5, Molecular Devices, USA)

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"While some plant compounds may indirectly influence GLP-1 pathways or mimic some GLP-1 effects, they are not equivalent to pharmaceutical GLP-1 receptor agonists," says Kumar

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