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After sugar depletion in the initial medium containing 4% (v/v) molasses and 5% (v/v) CSL, a feeding solution of 50% (v/v) molasses and 25% (v/v) CSL was continuously added to the bioreactor

The beads were washed three times with 1 ml of high-salt buffer (20 mM HEPES-KOH (pH 7.9), 500 mM KCl, 0.2 % NP-40, 15 % glycerol, 1 mM DTT) with rotation for 5 min at 4 C during each wash, followed by one detergent-free wash (20 mM HEPES-KOH (pH 7.9), 150 mM KCl, 15% glycerol, 1 mM DTT)

It depends on both copper and zinc as cofactors, but only copper regulates its activity ( 2 O 2 , CuZn-SODs activity is suppressed, via oxidation of His-118 to form 2-oxo-histidine ( Cytochrome c oxidase, also known as complex IV, at a final step in the respiratory chain, catalyzes the reduction of oxygen to water

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