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Plasma TNF concentration was colorimetrically measured using a commercially available enzyme-linked immunosorbent assay (ELISA) kit from R&D Systems (Minneapolis, MN)

The reaction mixture contained cell suspension (0.5 mL, 25 mg cells wet weight), substrate (0.5 mL, saturated aqueous solution for both DEHP and MEHP) and 2.5 mL of 50 mM phosphate buffer (pH 7.0)

Sigma) incorporated in the gels was photoactivated on a ChemiDoc (BioRad) using the stain-free gel application (590/110 filter and 45 s of activation)

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