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Then, the cell viability was determined, and 100 L supernatant from cell culture medium was collected to measure LDH content using the specific kit (Nanjing Jiancheng Bioengineering Institute, China) according to the manufacturers protocol [28]
Cytokines were categorized as either TH1 (pro-inflammatory: IL1, TNF, IFN) or TH2 (anti-inflammatory: IL4, IL5, IL10, IL13) to characterize distinct immune response patterns
J Inflamm Res 17:59856004
[34] [35] [36] [37] In August 2007, Capital One agreed to acquire NetSpend for $700 million
They may have delayed growth, failure to gain weight or developmental delays
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