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Protein (50 g) was separated using sodium dodecyl sulfate-polyacrylamide gel electrophoresis on a Precast PAGE Gel (412%, APPLYGEN, Beijing, China) and electroblotted onto nitrocellulose membranes

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J Biol Chem 273: 60096012 Zhang K, Kaufman RJ (2004) Signaling the unfolded protein response from the endoplasmic reticulum

1 mL syringe is preferred at 2.4 mg with 2.5 mg/mL since 0.5 mL syringes cap below the 0.96 mL draw