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Critically, this was achieved without requiring any carrier system, unlike TGF- and other growth factors that require special delivery mechanisms

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After purification, islets were cultured overnight at 37 C in a humidified atmosphere of 95% air and 5% CO 2 in RPMI 1640 medium supplemented with 10% FBS, 100 units/ml penicillin and 0.1 mg/ml streptomycin

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