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The membrane was then incubated at room temperature in blocking solution for 1 hour, followed by incubation in blocking solution containing the appropriate dilution of either phospho-Jak2 or the primary antibody for growth hormone receptor, for 2 hours

Herkenham M, Lee HY, Baker RA

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Quality and handling matter just as much as choosing the correct type, since improperly stored or handled water regardless of type can compromise sterility