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Thiol-containing compounds (RSH) react with DTNB to form mixed disulfides (RS-S-DTNB), where the thiol group replaces one of the nitrobenzoic acid moieties in DTNB

Orihuela D, Meichtry V, Pregi N, Pizarro M (2005) Short-term oral exposure to aluminium decreases glutathione intestinal levels and changes enzyme activities involved in its metabolism

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To further validate the multivalent interactions of D-CuP, SPR analysis was performed to quantify its binding ability to p53 and HDAC7 in comparison with M-CuP

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