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These pathway-level predictions were experimentally validated, as PYC significantly suppressed IL-6 and IL-1 expression in lipopolysaccharide-induced BV2 microglial cells, supporting its multi-component, multi-target mode of action ( Although in vitro studies have provided substantial evidence of anti-inflammatory activity, corresponding evaluations using in vivo models remain limited

The problem is twofold: 1

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Notably, the upregulation of ZIP14 and FPN acts a significant role in this process[143]

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