Evidence shows that certain 5-HT2A agonists with psychedelic properties, such as (R)-DOI (in primary rat aortic smooth muscle cells stimulated with TNF-), can inhibit NF-B nuclear translocation and suppress TNF--induced inflammatory gene expression in vitro, including ICAM-1, VCAM-1, and IL-6
To permeabolize the cell membrane, aspirate the PBS and add 300 l of 1% NP-40 in PBS (vol/vol,) for 10 min at room temperature
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Lachin JM, Bebu I, Nathan DM
How the FDA Classifies Peptides Not all peptides are subject to the same level of evidence, oversight, or regulation