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The HBSS was removed, and the cells washed four times with ice cold HBSS (pH 7.4), before fresh growth medium without FCS and supplemented with MEM non-essential amino acids (ThermoFisher) was added to one of the plates and incubated ON before samples were collected (recycling fraction)

After reconstitution, refrigerate them at 28C and use within the recommended timeframe

It also helps stimulate hair follicles and scalp health

Cells were then incubated in 0.04 M HCl in isopropanol and plates were gently shaken for 10 min at room temperature

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