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JCI Insight , 5(17), e140532

Supernatants were separated from the precipitate to quantify the non-complexed EXE fraction by HPLC and further calculate the precipitation efficiency as described below: Precipitates were freeze-dried (LyoBeta Mini, Telstar, Elancourt, France) using the following parameters: samples were frozen at 45 C with a temperature ramp of 1 C/min, then sublimated at 30 C and 0.05 mbar for at least 24 h, and finally underwent desorption under maximum vacuum at 10 C for 2 h

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Sections of 46 m were cut with a rotatory microtome and put on clean, dry glass slides