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After that, 1 ml diethyl ether was added to the samples to extract SCFAs, then samples were centrifuged at 14,000 g , 4 C, 15 min, and the supernatant was used for GC-MS 55

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Next, we examined whether livers from liver-specific Gclc KO mice induced an antioxidant response and had alterations in lipids and lipid-generating enzymes as initially observed in whole-body Gclc KO mice