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5 Endocrinology, Diabetology and Medical Andrology Unit, IRCCS, Humanitas Research Hospital, Milan, 20089, Italy Correspondence: Alessandra Di Maria, Department of Ophthalmology, IRCCS Humanitas Research Hospital, Rozzano, Milan, 20089, Italy, Tel +39 02 8224 2555, Email [email protected] Purpose: Dry eye disease (DED) is a prevalent ocular surface disorder frequently associated with type 2 diabetes mellitus (T2DM)

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Flow cytometry quantification of cell viability of neutrophils (n = 5) cultured in vitro with serum in the presence or absence of anti-IFNAR (10 g ml -1 ), or IgG depletion, for 16 hours

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