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qPCR validation analyses TotalRNA from each tissue was aliquoted and frozen at -80 C, and 2 g of total RNA was reverse-transcribed into cDNA using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Catalog # 4368813)

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doi: 10.1093/jn/132.8.2361S 228 Kazmierczak-Bar NskaKKarwowskiJ;KarmBT;HalczukKKazmierczakkazmierczak-Bar NskaJKarwowskiBTet al

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