Twenty-four hours later, medium was collected and stored at 20 C for further analysis, and cells were harvested, rinsed twice with ice-cold PBS, and lysed with RIPA buffer (140 mM NaCl, 20 mM Tris, pH=7.4, 10% glycerol, 1% Triton X-100, 0.5% deoxycholate, 0.1% SDS, 2 mM EDTA, 1 mM phenylmethylsulfonyl fluoride and leupeptin at 20 g ml 1 )
Lucchinetti, C
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This interaction triggers an adaptive immune response, leading to the activation of gluten-specific T-cells and the release of pro-inflammatory cytokines, which cause intestinal damage
Product glucuronides are exported from hepatocytes by MRP2 (canalicular, into bile) and MRP3/MRP4 (basolateral, into blood for renal excretion)