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For immunohistochemical labeling of 17-hydroxysteroid dehydrogenase (HSD17B) Bouins solution-fixed testes section were deparaffinized, rehydrated, blocked by incubation with 1% rabbit serum in phosphate buffered saline (PBS) and incubated with rabbit anti-HSD17B antibody (sc-31,620, Santa Cruz Biotechnology, Dallas, TX) diluted to 4 g/mL in 1% rabbit serum in PBS in a humidified chamber overnight at 4 C