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Colonies formed in each well were fixed, stained with crystal violet and numbered

The complexes induce endoplasmic reticulum (ER) stress by inhibiting ubiquitin-proteasome system (UPS) activity, leading to polyubiquitinated protein accumulation and activation of the unfolded protein response (UPR)

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They can inhibit therapeutic efficacy by reducing intracellular concentration, decreasing its bioavailability, and reducing the transition through the bloodbrain barrier (BBB)