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A dilution series of cysteinyl-glutathione (CSSG, Nereithione) was prepared using sterile seawater from the sites (0.2 m filtered) at 10 6 M to 10 9 M concentrations and added to the swimming worms using a micropipette until male worms were initiated to swim in narrow circles and release sperm as described by Results and discussion Animal behavior, especially when observed in the field, is complex to interpret, as a range of environmental factors that are difficult to control and, in many publications, not even determined or reported ( Figure 1 ), impact organisms responses
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