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2016;2(5):e1600200

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Through these mechanisms, GPX4 establishes the redox threshold that separates cell survival from lipid peroxidation-induced death, functioning as the master regulator of ferroptosis

After denaturing in a boiling water bath, the proteins were separated by SDSpolyacrylamide gel electrophoresis (SDSPAGE), transferred to a PVDF membrane, blocked with 5% milk in TBST for 30 min at room temperature, and then incubated with primary antibody at 4 C overnight with shaking