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MtDNA mutations, which accumulate with age due to replication errors and ROS damage exceeding repair capacity [15], are associated with age-related diseases [16, 17]

To validate their ability to differentiate into osteogenic and adipogenic lineages, the expression of RUNX-2, ALP, FABP4, and PPAR were evaluated using real time polymerase chain reaction (RT-PCR) as reported [43]

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