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The only question was when

Unlike AAV vectors, which provoke immunological responses after the second administration, LNPs are expected to allow repeated administrations 37,38

It is necessary to control the temperature, to reduce sample volume to shorten the analysis time and the mixing has to be instantaneous

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The water-soluble nature of B12 means several things for patients : the vitamin requires regular dietary intake to maintain optimal levels, it can be lost during food preparation (particularly with prolonged cooking or boiling), and therapeutic doses are generally considered safe as excess is eliminated through the kidneys, though the body's substantial hepatic storage also plays a role in B12 metabolism