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The peptide comes as lyophilized powder requiring reconstitution with bacteriostatic water before use

The antiallodynic effect was entirely blocked by the microglial inhibitor minocycline, -endorphin antiserum, or the opioid receptor antagonist naloxone [24]

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After transfection for 48 h, cells were treated with metformin (1.0 mmol/L) for 2 h, followed by the PA exposure for additional 22 h

So there are technical limitations with the Nourish platform, and for the best possible outcomes, you may have to put time into adjusting data points where you see fit