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Step 4: Preparation of compound of formula-13 The reaction mixture obtained in step 3 was slowly added to a mixture of ethanol (850 ml), DIPEA (64 ml) and 2-(2-(2-aminoethoxy)ethoxy)acetic acid compound of formula- 10 (50 gm) at 25-30C and stirred the obtained reaction mixture for 45 min at the same temperature

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After resolved in SDS-PAGE, the immunoprecipitates were transferred onto polyvinylidene fluoride membranes (Whatman, GE Healthcare, NJ, USA)

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Some patients who have already lost weight appear non-adherent because they reduce the frequency of injections