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This could be related to very low leptin levels in this model of CGL

Gallbladder motility: Extreme calorie restriction can reduce gallbladder contractions, allowing bile to stagnate and form stones

423 The study began by inducing inflammation in mice through the injection of various Toll-like receptor (TLR) agonists and then assessed the inflammation by measuring plasma tumor necrosis factor-alpha (TNF-) levels

Gene expression levels were quantified by qPCR using QuantiFast SYBR Green PCR Kit (Qiagen, Chatsworth, CA, USA) and reactions were performed on a Viia TM 7 real time PCR system (Life Technologies, Gaithersburg, MD, USA)

How Does GLP-1 Function in Various Organs