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Currently, the gene editing strategies for live bacteria mainly include CRISPR/Cas9 technology, zinc finger nuclease (ZFN) technology, and transcription activator-like effector nuclease (TALEN) technology

(2) Mar M, Morales A, Colell A, Garca-Ruiz C, Fernndez-Checa JC

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We determined cell viability and cytotoxicity by using tetrazolium reduction assay and Lactate Dehydrogenase (LDH) assay, performed real-time PCR and protein quantification to assess the expression of biomarkers after treating cells with copper peptide (GHK-Cu), copper chloride (CuCl 2 ) and copper acetate (Cu(OAc) 2 )