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The closest comparison is with AHK-Cu (Ala-His-Lys with copper), a related copper-binding tripeptide that shares the histidine-anchored coordination chemistry but has alanine rather than glycine at position 1

Gene expression levels were standardized using the z score method, and cluster analysis of DEGs in key pathways was performed using the pheatmap R package [33] based on standardized FPKM values